CURRENT POSITION

Associate Professor, Department of Clinical Veterinary Medicine, College of Veterinary Medicine, South China Agriculture University, China 

MAJOR INTEREST

Equine Veterinary Education, 

Equine infectious diseases, 

EDUCATION

PhD, Veterinary Science, Gluck Equine Research Center, University of Kentucky. 

Equine Immunology. Mentor: Dr. David W. Horohov, August 2007 - December 2012.

MPVM, Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, China. Veterinary Immunology and Microbiology. Mentor:  Dr. Junwei Wang, September, 2003 - July 2006.

B.Ag. Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, China. Veterinary Medicine. September, 1999 - July 2003.

Graduate student, Preparing for Future Faculty (PFF), Graduate school, University of Kentucky. College Teaching and Learning. Mentor: Dr. Morris A. Grubbs. August 2010- December 2012.      

EXPERIENCE

College of Veterinary Medicine

Director, Section of Veterinary Surgery and Obstetric, Department of Clinical Veterinary Medicine, College of Veterinary Medicine, South China Agriculture University, Guangzhou, Guangdong, China. January 2016 – till now.

College of Veterinary Medicine

Associate Professor, Department of Clinical Veterinary Medicine, College of Veterinary Medicine, South China Agriculture University, Guangzhou, Guangdong, China. January 2013 – till now. 

Gluck Equine Research Center

Research Assistant, Department of Veterinary Science, University of Kentucky, Lexington, KY, August 2007 – December 2012.  

OIE Laboratory Reference for Equine Influenza 

Research Volunteer, Sep. 2006-Dec. 2006.  

China Highly Pathogenic Avian Influenza Prevention and Control Headquarters, Branch of Science and Technological in Heilongjiang province

Research Assistant, February 2004 - April 2004

Department of Preventive Veterinary Medicine 

Research Assistant, College of Veterinary Medicine, Northeast Agriculture University, Septmber2003 – July 2006.  

Collage of Veterinary Medicine

Research Volunteer, Department of Preventive Veterinary Medicine, Northeast Agriculture University, September 2002 – May 2003.  

PROJECTS

Mechnism of antigen presenting cells in foal’s resistance to Rhodococcus equi infection (No. 31402259), the National Natural Science Fund, 2015.1-2017.12. Principle Investigator.

Susceptibility of horse to infectious diseases and DNA methylation (No. S201304001545), Natural Science Fund of Guangdong Province, 2013.10-2015.10, Principle Investigator.

Environmental effect on immunological responses of horses to Equine Influenza Virus (ranking top 4, still under verification), Guangdong Province Science and Technology Plan of Foreign Cooperation Projects,  2014.01-2016.12, Principle Investigator.

The function of Cfa-miR-143 in pathogenesis of canine influenza infection (S2013020013858), Natural Science Fund of Guangdong Province, responsible for analysis of cell signaling.

Environment effect on respiratory immune development in foals including antigen presenting cells and lymphocytes (supported by USDA 2010-65119-20600), principle person who completed the major part of the project - evaluation of environmental effect on Dendritic cells and macrophages of foals (My Ph.D. dissertation). 

Epigenetic regulation of IFN expression in foals (supported by USDA 2010-65119-20600), principle person who completed the major part of the project –regulation of DNA methylation on IFN expression in foals (My Ph.D. dissertation). 

Immunity to Avian Influenza Virus infection in various animals (chickens, ducks, geese and pigs ) and the development of high through put detection techniques (Funded by NSFC, certificate No. 2004BA519A23), principle person who completed the major part of the project (My MPVM theses).

INTELLECTUAL PROPERTY

Patent: A test kit for detecting Rhodococcus equi, China Patent No. ZL 2015 1 0273815.1, September 28th 2016, Sun Lingshuang, Yuan Liguo, Li Shoujun,

Patent: The application of a recombinant protein VapA of a pathogenic gene from Rhodococcus equi, China Patent No. ZL 2015 1 0273207.0, December  26th 2017, Sun Lingshuang, Gong Fengping, Jia Kun, Li Shoujun, 

Patent: IgY+IgY(Delta Fe) (H+L) horseradish peroxidase marker antibody, China Patent No. CN1817905, August 16th 2006, Wang Junwei, Sun Lingshuang

Patent:IgY+IgY(delta Fc)(H+L) and preparation thereof, China Patent No. CN1818651, July 21st 2010,Wang Junwei, Sun Lingshuang 

TEACHING EXPERIENCE

Instructor, “Equine Diseases (VM301)”, “Large Animal Surgery (VM403)”, “Small Animal Surgery (VM407)”, “English for clinical Veterinary Medicine (VM604)”, College of Veterinary Medicine, South China Agriculture University.

Guest Lecturer, “immune-associated cells and molecules in the blood and bronchial alveolar lavage”, Introductory anatomy, physiology and animal hygiene (VS350), 2011 Summer, 

Department of Veterinary Science, University of Kentucky

Supervisor: Professor Roberta M. Dwyer.

Guest Lecturer, “Equine industry in China”, Topics in the Equine Industry (EQS309), 2010 Fall 

Midway Collage, Midway, KY 40347

Supervisor: Professor Kimberly Tumlin. 

Teaching Assistant: Introductory anatomy, physiology and animal hygiene (VS350), 2011 summer 

Assistant on exam generation and grading, and laboratory guidance 

Department of Veterinary Science, University of Kentucky

Supervisor: Professor Roberta M. Dwyer.

Graduate Instructor, Veterinary Immunology Laboratory (VM205), 2004 fall and 2005 spring 

Working on lecture and guidance for the immunological associated laboratory techniques

Department of Veterinary Medicine, Northeast Agriculture University, China

Supervisor: Professor Junwei Wang

INTERNATIONAL COOPERATIVE ACTIVITIES 

Scientific Committee member, International Symposium of One Health Research, Nov. 22-23, 2014. 

Interpreter & Chief organizer, Equine Leadership Summit, April 26th - May 5th 2012, Lexington KY. This project is to build a relationship between the official Chinese Equestrian Association and equine sports industry in Kentucky. Participants including Governor of Kentucky, University of Kentucky, Kentucky Horse Park, Keeneland, ect. 

Volunteer & Translator, 2010 World Equestrian Game, September – October 2010  

Coordinative Assistant & Translator, Sino - America Dairy Collaborative Project, “A Comprehensive Program to Increase the Productivity of Dairy Farms in Heilongjiang Province”, 2008-2010. This project is supported and funded by Heilongjiang Provincial Department of Science & Technology, China. 

FELLOWSHIP AND AWARDS

Certificate in One Health, August 2014.

Wright Markey Trust Fund, August 2007-Decemenber 2012

University of Kentucky Graduate Student Travel Fund, 2008, 2009, 2010, 2011, 2012

First Grade Scholarship of Northeast Agriculture University, 2002

First Grade Scholarship of Northeast Agriculture University, 2001

Da Bei Nong Scholarship (certificate No. DBND2000-009), 2000

MEMBERSHIP 

Councilor of Guangdong Provincial Equine Industry Association 

China Equestrian Association

American Association of Equine Practitioner

American Association of Immunologists

American Association of Veterinary Immunologist

University of Kentucky Alumni Association 

PUBLICATIONS

Selected peer-reviewed articles

1.Gang Lu, Lingshuang Sun, Tao Xu, Dong He, Zengchao Wang, Shudan Ou, Kun Jia, Liguo Yuan, Shoujun Li, First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang, Province and Hong Kong District, Plos One, 2016 May 16.（同等贡献）

2.Lingshuang Sun, Lifang Wang, Weidong Zhang, Yankuo Sun, Yongfeng Luo, Yao Sun, Ziguo Yuan, Heng Wang, Zhangyong Ning, Kun Jia, and Li-guo Yuan. Lack of Exposure of H10N8 Avian Influenza Virus Among Veterinarians in Guangdong Province, China. Journal of Medical Virology 2015 May 15. doi: 10.1002/jmv.24268.( SCI，IF 2.347，4区)

3.Lingshuang Sun, Pei Zhou, Shuyi He, Yongfeng Luo, Kun Jia, Cheng Fu, Yao Sun, Huamei He, Liqing Tu, Zhangyong Ning, Ziguo Yuan, Heng Wang, Shoujun Li, Liguo Yuan,. Sparse serological evidence of H5N1 avian influenza virus infections in domestic cats, northeastern China. Microbial Pathogenesis. 82 (2015) 27-30. (2.0，四区)

4.LS Sun, LF Wang, ZY Ning, ML Hong,Y Zheng, SY He, LQ Tu, G Lu, HT Li, H Wang, WB Qi, LG Yuan and SJ Li, Lack of Evidence of Avian-to-Cat Transmission of Avian H5 subtype Influenza Virus among Cats in Southern China, Pak. Vet. J. 2014 Vol. 34 No. 4 pp. 535-537. 

5.Jia K, Jiao G, Hong ML, Sun Y, He HM, Ning ZY, Wang H, Yuan ZG, Zhou P, Li SJ, Sun L,. No evidence H10N8 avian influenza virus infections among poultry workers in Guangdong Province before 2013. Journal of Clinical Virology. 2015 Jan; 62:6-7. doi: 10.1016/j.jcv.2014.11.012. Epub 2014 Nov 15. 

6.Zhou H, He SY, Sun L, He H, Ji F, Sun Y, Jia K, Ning Z, Wang H, Yuan L, Zhou P, Zhang G, Li S. Serological evidence of avian influenza virus and canine influenza virus infections among stray cats in live poultry markets, China. Vet Microbiol. 2015 Feb 25;175(2-4):369-73. doi: 10.1016/j.vetmic.2014.12.018. Epub 2014 Dec 27. （同等贡献，SCI，IF 2.726，1区）

7.Hua-tao Li, Ling-suang Sun, Zhong-ming Chen, Jing-si Hu, Cun-dong Ye, Kun Jia, Heng Wang, Li-guo Yuan, Gui-hong Zhang, Shoujun Li. Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification. The Veterinary Journal. 2014, 199 468–47. (同等贡献，2.165，一区)

8.Lingshuang Sun, Amanda A. Adams, Allen E. Page, Alejandra Betancourt, David W. Horohov. The effect of environment on interferon-gamma production in neonatal foals. Veterinary immunology and immunopathology 143(1-2):170-175, 2011. 

9.Sun, L. Gong, Z., Horohov, D.W., Methylation of the IFN- gene promoter is correlated with IFN- expression in foals. Journal of Equine Veterinary Science, 32, S29, 2012. 

10.Lingshuang Sun, Amanda A. Adams, Alejandra Betancourt, John C Stewart, Chong Liu, David W. Horohov. The role of proliferation in the regulation of IFN expression by foals. Developmental and Comparative Immunology. 36(3):534-539, 2012. 

11.Lingshuang Sun, Zhangbin Gong, Alejandra Betancourt, Amanda A. Adams, David, W. Horohov. The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression, Developmental t and Comparative Immunology, 39(3):273-278, 2013. 

12.Sun Lingshuang, Qi yan. Duck and Goose Immunoglobulins Purification from Egg Yolk and Their Comparison. (2007) 01-0010-02, Chinese Journal of Veterinary Science and Technology.

13.Sun Lingshuang; Qi Yan; Shi Nan; Wang Junwei. Purification and Comparison of Duck and Goose Immunoglobulins from Yolk. 2006, v19, Chinease Poultry.

14.Jia Shanshan, Sun Lingshuang, Qi Yan. Expression of HA1 gene of avian influenza virus H7 subtype in recombinant baculovirus and analysis of antigenicity of the expressed HA1 protein. v. 37 (3) p. 232 - 235, 2007, Veterinary Science in China.

15.Hansen S, Sun L, Baptiste KE, Fjeldborg J, Horohov DW. Age-related changes in intracellular expression of IFN-γ and TNF-α in equine lymphocytes measured in bronchoalveolar lavage and peripheral blood. Dev Comp Immunol. 2012 Dec 5. 

16.Sanz M, Loynachan A, Sun L, Oliveira A, Breheny P, Horohov DW.The effect of bacterial dose and foal age at challenge on Rhodococcus equi infection. Vet Microbiol. 2013 Dec 27;167(3-4):623-31. doi: 10.1016/j.vetmic.2013.09.018. Epub 2013 Sep 24.

17.Wei C, Huang Z, Sun L, Xie J, Chen Y, Zhang M, Zhang C, Qi H, Qi W, Ning Z, Yuan L, Wang H, Zhang L, Zhang G. Expression and Antibody Preparation of GP5a Gene of Porcine Reproductive and Respiratory Syndrome Virus. Indian J Microbiol. 2013 Sep;53(3):370-5. doi: 10.1007/s12088-013-0368-1. Epub 2013 Mar 2. 

18.Qi Yan, Sun Ling-shuang, Wang Bin, Sun Jin-hua, Wang Jun-wei, Shi Dong-fang. Eukaryotic expression of HA1 gene of H5 avian influenza virus in insect cells and the antigenicity of the expressed protein. 2006 36(5). Veterinary science in China.

19.Qi Yan, Sun Lingshuang, Zhang Henan, Wang Bin, Qu Zhehui, Shi Nan, Shi Dongfang, Wang Junwei. Preliminary comparative analysis on HA and HA1 expressed in Sf-9 insect cells of H5 subtype AIV as detective antigen. 2008 39(11). Journal of northeast agricultural university. 

20.Liu C, Betancourt A, Cohen DA, Adams AA, Sun L, Horohov DW.Vet Immunol Immunopathol. Granzyme B-mRNA expression by equine lymphokine activated killer (LAK) cells is associated with the induction of apoptosis in target cells.2011 Sep 15;143(1-2):108-15. doi: 10.1016/j.vetimm.2011.06.031. Epub 2011 Jul 13. 

21.Su S, Zhou P, Fu X, Wang L, Hong M, Lu G, Sun L, Qi W, Ning Z, Jia K, Yuan Z, Wang H, Ke C, Wu J, Zhang G, Gray GC, Li S. Virological and epidemiological evidence of avian influenza virus infections among feral dogs in live poultry markets, china: a threat to human health? Clin Infect Dis. 2014 Jun;58(11):1644-6. doi: 10.1093/cid/ciu154. Epub 2014 Mar 12. 

22.Su S, Chen J, Jia K, Khan SU, He S, Fu X, Hong M, Sun L, Qi W, Gray GC, Li S. Evidence for subclinical influenza A(H1N1)pdm09 virus infection among dogs in Guangdong Province, China. J Clin Microbiol. 2014 May;52(5):1762-5. doi: 10.1128/JCM.03522-13. Epub 2014 Mar 5. 

23.Zhou H, Zheng Y, Wang L, Cui J, Fu X, He S, Sun L, Jia K, Zhou P, Qi SW, Zhang G. First serologic study for influenza A (H7N9) virus among veterinarians in Guangdong, China. J Clin Virol. 2014 Jun;60(2):182-3. doi: 10.1016/j.jcv.2014.03.007. Epub 2014 Mar 18. No abstract available. 

24.Zha Y, Xie J, Chen Y, Wei C, Zhu W, Chen J, Qi H, Zhang L, Sun L, Zhang X, Zhou P, Cao Z, Qi W, Zhang M, Huang Z, Zhang G. Microbiological identification and analysis of Swine lungs collected from carcasses in Swine farms, china. Indian J Microbiol. 2013 Dec;53(4):496-8. doi: 10.1007/s12088-013-0396-x. Epub 2013 Mar 27. 

25.Zhou H, Cao Z, Tan L, Fu X, Lu G, Qi W, Ke C, Wang H, Sun L, Zhang G. Avian-like A (H1N1) swine influenza virus antibodies among swine farm residents and pigs in southern China. Jpn J Infect Dis. 2014;67(3):184-90. 

26.Tong S, Tian J, Wang H, Huang Z, Yu M, Sun L, Liu R, Liao M, Ning Z. H9N2 avian influenza infection altered expression pattern of phiogosine-1-phosphate receptor 1 in BALB/c mice. Virol J. 2013 Sep 30;10:296. doi: 10.1186/1743-422X-10-296. 

27.Wang H, Jia K, Qi W, Zhang M, Sun L, Liang H, Du G, Tan L, Shao Z, Ye J, Sun L, Cao Z, Chen Y, Zhou P, Su S, Li S. Genetic characterization of avian-origin H3N2 canine influenza viruses isolated from Guangdong during 2006-2012. Virus Genes. 2013 Jun;46(3):558-62. doi: 10.1007/s11262-013-0893-3. Epub 2013 Mar 6. 

Selected Abstracts

1.Lingshuang Sun, A.A. Adams, D.W. Horohov. Regulation of interferon-gamma (IFNγ) gene expression in foals by proliferation and DNA methylation. Poster presentation during 89th Conference of Research Worker in Animal Diseases, Chicago, Illinois, USA, 2008.

2.Lingshuang Sun, Amanda A. Adams, Eric J. Oberst, Daisy M. Ramos, David W. Horohov. The effect of environment on interferon-gamma (IFNγ) production in neonatal foals.  Oral presented at 90th Conference of Research Worker in Animal Diseases, Chicago, Illinois, USA, 2009.

3.Lingshuang Sun, Alejandra Betancourt, Allen Page, Eric J. Oberst, Amanda A. Adams, David W. Horohov. Environment affects interferon-gamma (IFN production in neonatal foals through possible effects on dendritic cells.  Oral presented at 91st Conference of Research Worker in Animal Diseases, Chicago, Illinois, 2010.

4.Lingshuang Sun, Zhangbin Gong, David W. Horohov.  The DNA promoter of the interferon gamma gene (Ifng) is hypermethylated in neonatal foals.  Oral presentation at 92nd Conference of Research Worker in Animal Diseases, Chicago, Illinois, USA, 2011.

5.L. Sun, Z. Gong, D. W. Horohov, The methylation level of the IFN gene promoter is correlated with IFN expression in foals.  International Conference on Equine Infectious Diseases IX, Oct. 21-26 in Lexington, KY, USA ,2012.

6.Lingshuang Sun, Zhangbin Gong, Eric J. Oberst,  David W. Horohov.  The hypermethylation of IFNg gene promoter is correlated with IFNg deficiency in the neonatal foals and its demethylation with aging is associated with environment.  Oral presentation at 5th Havemeyer Workshop on Rhodococcus equi, Deauville, France, 11th of July, 2012. 

7.Lingshuang Sun, Macarena G. Sanz, Alan T. Loynachan, Whitney Zoll, Allen Page, David W. Horohov. Age-related susceptibility to R equi infection in foals. Oral presented presentation at 93th Conference of Research Worker in Animal Diseases, Chicago, Illinois, USA, 2012.

8.David W. Horohov, Lingshuang Sun, Age-dependent susceptibility of young foals to Rhodococcus equi. Presentation at 5th Havemeyer Workshop on Rhodococcus equi, Deauville, France, 11th of July, 2012.

9.Zhangbin Gong, Lingshuang Sun, David W. Horohov.  A specific CpG site demethylation in the IFN-gamma gene promoter region of different aged equine.  Presented during 92nd Conference of Research Worker in Animal Diseases, Chicago, Illinois, USA, 2011. 

10.Shoujun Li, Lingshuang Sun. Canine Influenza Infection in China. Oral presentation at the 3rd International Symposium on Neglected Influenza Viruses. Athens, Georgia, USA, 2015.

11.Fengping Gong, Lingshuang Sun*. Rhodococcus equi infection in China. Journal of Equine Veterinary Science 39:S87. DOI: 10.1016/j.jevs.2016.02.186.