目录
2015.1-2015.11 密歇根大学博士后
2011.7-2014.12 中国科学院广州生物医药与健康研究院 助理研究员/副研究员
2004.9-2007.7 华南理工大学 生物化学与分子生物学 硕士
1999.9-2003.7 华南理工大学 生物工程 学士
主要研究项目包括：（1）猪基因编辑技术的研究和基因编辑猪的培育；（2）猪抗病育种研究；（3）种猪生物育种，育种新材料的创制与品种培育；（4）猪CRISPR筛选文库及猪功能基因的高通量筛选；（5）猪精原干细胞及相关代精和繁殖技术的开发。
国家自然科学基金面上项目（32372874）：基于“代精公猪“受体的精原干细胞移植研究，2024.01-2027.12，主持
国家自然科学基金面上项目（31772555）：基于精原干细胞移植技术制作基因编辑猪，2018.01-2021.12，主持
国家自然科学基金青年项目（31301103）：以CRISPR/Cas9系统制备基因敲除猪，2014.01-2016.12，主持
广东省科技计划项目（2017B020201009）：基于基因编辑技术的抗流行性腹泻病毒基因敲除猪的制备，2017.1-2019.12，主持
中科院重点部署项目（KSZD-EW-Z-005-003-002）：具有优良经济性状的基因修饰家畜的建立 子课题，2013.01-2015.12，主持
广东省科技厅，企业联合基金-重点项目（2019B1515210030）：重要猪源病毒感染相关宿主蛋白的鉴定及其在抗病育种中的应用，2020.01-2022.12，主持
Xin Zhao, Weican Wan, Bin Li, Xianyu Zhang, Mao Zhang, Zhenfang Wu*, Huaqiang Yang*. Isolation and in vitro expansion of porcine spermatogonial stem cells. Reproduction in Domestic Animals. 2022, 57(2):210-220.
Xin Zhao, Weican Wan, Xianyu Zhang, Zhenfang Wu*, Huaqiang Yang*. Spermatogonial Stem Cell Transplantation in Large Animals. Animals. 2021, 11(4):918.
Huaqiang Yang, Zhenfang Wu. Genome editing of pigs for agriculture and biomedicine. Frontiers in Genetics, 2018, 9:0-360.
Xianyu Zhang, Xin Zhao, Guoling Li, Mao Zhang, Pingping Xing, Zicong Li, Bin Chen*, Huaqiang Yang*, Zhenfang Wu*. Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation, Biology Open, 2020, 10(1):1-8.
Mao Zhang, Xin Zhao, Hongyi Li, Gengyuan Cai, Huaqiang Yang*, Zhenfang Wu*. Intratesticular injection of busulfan for producing recipient male pigs for spermatogonial stem cell transplantation. Livestock Science, 2021, 245:104448.
Yu C, Zhong H, Yang X, Li G, Wu Z*, Yang H*. Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells. Biotechnol J. 2021 Oct 27:e2100408. doi: 10.1002/biot.202100408.
Li L, Meng H, Zhang J, Liu Y, Zou Q, Gao Y*, Yang H*, Lai L*. A tunable, rapid, and precise drug control of protein expression by combining transcriptional and post-translational regulation systems. J Genet Genomics. 2020 Nov 20;47(11):705-712. doi: 10.1016/j.jgg.2020.07.009.
Huang Y, Li Z, Song C, Wu Z*, Yang H*. Resistance to pseudorabies virus by knockout of nectin1/2 in pig cells. Arch Virol. 2020 Dec;165(12):2837-2846. doi: 10.1007/s00705-020-04833-x.
Li G, Zhang X, Wang H, Liu D, Li Z, Wu Z*, Yang H*. Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors. Int J Biochem Cell Biol. 2020 Aug;125:105790. doi: 10.1016/j.biocel.2020.105790.
Zhang J, Wu Z, Yang H*. Aminopeptidase N Knockout Pigs Are Not Resistant to Porcine Epidemic Diarrhea Virus Infection. Virol Sin. 2019 Oct;34(5):592-595. doi: 10.1007/s12250-019-00127-y.
Li G, Zhang X, Ou H, Wang H, Liu D, Yang H*, Wu Z*. PIK-75 promotes homology-directed DNA repair. J Genet Genomics. 2019 Mar 20;46(3):141-144. doi: 10.1016/j.jgg.2019.03.002.
Li L, Meng H, Zou Q, Zhang J, Cai L, Yang B, Weng J, Lai L*, Yang H*, Gao Y*. Establishment of gene-edited pigs expressing human blood-coagulation factor VII and albumin for bioartificial liver use. J Gastroenterol Hepatol. 2019 Oct;34(10):1851-1859. doi: 10.1111/jgh.14666.
Zhao C, Shi J, Zhou R, He X, Yang H*, Wu Z*. DZNep and UNC0642 enhance in vitro developmental competence of cloned pig embryos. Reproduction. 2018 Apr 1;157(4):359-369. doi: 10.1530/REP-18-0571.
Zhang M, Cai G, Zheng E, Zhang G, Li Y, Li Z, Yang H*, Wu Z*. Transgenic pigs expressing β-xylanase in the parotid gland improve nutrient utilization. Transgenic Res. 2019 Apr;28(2):189-198. doi: 10.1007/s11248-019-00110-z.
Zhang X, Li Z, Yang H, Liu D, Cai G, Li G, Mo J, Wang D, Zhong C, Wang H, Sun Y, Shi J, Zheng E, Meng F, Zhang M, He X, Zhou R, Zhang J, Huang M, Zhang R, Li N, Fan M, Yang J, Wu Z. Novel transgenic pigs with enhanced growth and reduced environmental impact. Elife. 2018 May 22;7:e34286. doi: 10.7554/eLife.34286.
Li G, Liu D, Zhang X, Quan R, Zhong C, Mo J, Huang Y, Wang H, Ruan X, Xu Z, Zheng E, Gu T, Hong L, Li Z, Wu Z*, Yang H*. Suppressing Ku70/Ku80 expression elevates homology-directed repair efficiency in primary fibroblasts. Int J Biochem Cell Biol. 2018 Jun;99:154-160. doi: 10.1016/j.biocel.2018.04.011.
Yan S, Tu Z, Liu Z, Fan N, Yang H, Yang S, Yang W, Zhao Y, Ouyang Z, Lai C, Yang H, Li L, Liu Q, Shi H, Xu G, Zhao H, Wei H, Pei Z, Li S, Lai L, Li XJ. A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington's Disease. Cell. 2018 May 3;173(4):989-1002.e13. doi: 10.1016/j.cell.2018.03.005.
Yang H, Zhang J, Zhang X, Shi J, Pan Y, Zhou R, Li G, Li Z, Cai G, Wu Z. CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus. Antiviral Res. 2018 Mar;151:63-70. doi: 10.1016/j.antiviral.2018.01.004.
Li G, Zhang X, Zhong C, Mo J, Quan R, Yang J, Liu D, Li Z, Yang H*, Wu Z*. Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells. Sci Rep. 2017 Aug 21;7(1):8943. doi: 10.1038/s41598-017-09306-x.
Yang H, Wang G, Sun H, Shu R, Liu T, Wang CE, Liu Z, Zhao Y, Zhao B, Ouyang Z, Yang D, Huang J, Zhou Y, Li S, Jiang X, Xiao Z, Li XJ, Lai L. Species-dependent neuropathology in transgenic SOD1 pigs. Cell Research. 2014, 24(4):464-81.
Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H*, Lai L*. Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Cell Mol Life Sci. 2015, 72(6):1175-84. (*corresponding authors)
Huang J, Guo X, Fan N, Song J, Zhao, Zhen B, Ouyang Z, Liu Z, Zhao Y, Yan Q, Yi X, Schambach A, Frampton J, Esteban M, Yang D, Yang H*, Lai L*. RAG1/2 Knockout Pigs with Severe Combined Immunodeficiency. J Immunol. 2014, 193(3):1496-503. (*corresponding authors)
Wang G*, Yang H*, Yan S*, Wang CE, Liu X, Zhao B, Ouyang Z, Yin P, Liu Z, Zhao Y, Liu T, Fan N, Guo L, Li S, Li XJ, Lai L. Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain. Molecular Neurodegeneration 2015, 10:42. (*Equal contribution)
Yang D*, Yang H*, Li W, Zhao B, Ouyang Z, Liu Z, Zhao Y, Fan N, Song J, Tian J, Li F, Zhang J, Chang L, Pei D, Chen YE, Lai L. Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning. Cell Res. 2011, 21(6):979-82. (*Equal contribution)
Wang Y*, Yang HQ*, Jiang W, Fan NN, Zhao BT, Ou-Yang Z, Liu ZM, Zhao Y, Yang DS, Zhou XY, Shang HT, Wang LL, Xiang PY, Ge LP, Wei H, Lai LX. Transgenic expression of human cytoxic T-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) by porcine skin for xenogeneic skin grafting. Transgenic Res. 2015, 24(2):199-211. (*Equal contribution)
